货号 | CAS号 | 编号 | 包装 | 参数 | 库存 | 补货期 | 价格 |
BK2021081201 | CAS | BK2021081201 | 100 | 0 |
Name:Streptavidin(链霉亲和素)
Description:Streptavidin Recombinant (重组链霉亲和素)
Pricings:5mg (RMB 300.00), 20mg (RMB 780.00), 1g (RMB 21000.00)
Shipped:At Room Temperature
Catalogue Number:PRO-rSA001
INTRODUCTION
Streptavidin is a tetrameric protein secreted by Streptomyces avidinii which binds firmly to biotin. Streptavidin is widely used in molecular biology through its unique high affinity for the vitamin biotin. The dissociation constant (Kd) of the biotinstreptavidin complex is about ~1015mol/L. The strong affinity recognition of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits. The streptavidin/biotin system has one of the biggest free energies of association of yet observed for noncovalent binding of a protein and small ligand in aqueous solution (K_assoc = 1014). The complexes are also extremely stable over a wide range of temperature and pH.
DESCRIPTION
StreptavidinStreptomyces AvidiniiRecombinant produced in E.Coli. The molecular weight per tetramer is approximately 52kDa.
SOURCE
Escherichia Coli.
PHYSICAL APPEARANCE
Sterile Filtered White lyophilized (freezedried) powder.
FORMULATION
Lyophilized in 10mM potassium phosphate buffer pH 6.5.
SOLUBILITY
It is recommended to reconstitute the lyophilized Streptavidin in sterile 18MΩ-cm H2O not less than 0.5 mg/ml, which can then be further diluted to other aqueous solutions.
STABILITY
Streptavidin is shipped at ambient temperature, upon arrival store at 20 ℃.
PURITY
Greater than 98.0% as determined by SDS-PAGE and HPLC.
SPECIFIC ACTIVITY
17U/mg (one unit binds 1 μg Dbiotin at pH 8.9).
PROTEOLYTIC ACTIVITY
10 U/mg protein (25 °C, 24 h, pH 8.0).
AMINO ACID SEQUENCE
MAEAGITGTWYNQLGSTFIVTAGADGALTGTYESAVGNAESRYVLTGRYDSAPATDGSGTALGWTVAWKNNYRNAHSATTWSGQYVGGAEARINTQWLLTSGTTEANAWKSTLVGHDTFTKVKPSAAS.
USAGE
PuriMag’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
采用引用该产品相关文献
1. Hailiang Sun, Jiate Lin, Zhiting Liu, Yanan Yu, Meihua Wu, Shuo Li, Yang Liu, Yaling Feng, Yuqian Wu, Mingliang Li, Peirong Jiao, Kaijian Luo and Ming Liao. Genetic, Molecular, and Pathogenic Characterization of the H9N2 Avian Influenza Viruses Currently Circulating in South China, Viruses 2019, 11(11), 1040(链霉亲和素包覆多孔板)
2. Yang Liu, Shuo Li, Huapeng Sun, Liangqi Pan, Xinxin Cui, Xuhui Zhu, Yaling Feng, Mingliang Li, Yanan Yu, Meihua Wu, Jiate Lin, Fengxiang Xu, Shaohua Yuan, Shujian Huang, Hailiang Sun and Ming Liao. Variation and Molecular Basis for Enhancement of Receptor Binding of H9N2 Avian Influenza Viruses in China Isolates, Front. Microbiol., 17 December 2020, #(链霉亲和素包覆多孔板)
3. Meihua Wu, et al. Molecular Characteristics, Antigenicity, Pathogenicity, and Zoonotic Potential of a H3N2 Canine Influenza Virus Currently Circulating in South China, Front. Microbiol., 09 March 2021, doi: 10.3389/fmicb.2021.628979(链霉亲和素包覆多孔板)
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