■产品描述

G-streptavidin链霉亲和素磁珠通过链霉亲和素和生物素之间的高亲和力作用，可以绑定任何生物素标记的分子，如抗体、蛋白质、多肽、DNA等。粒子具有较大的比表面积和很高的捕获效率。 G-streptavidin-biotinbiomolecule复合物通过磁分离易于从溶液中分离，因此提供了一个快速、简洁的磁性纳米粒子标记的生物分子的方法。链霉亲和素磁珠可用于各种下游分离过程：蛋白纯化、细胞分离、免疫及分子检测。链霉亲和素-生物素（SA-Biotin） 系统具有极高的结合亲和力（K =10^-15） ， 在生物领域具有广泛的应用。本产品采用超顺磁性微球，粒径均一、比表面积大、形貌规整，有利于方便、快捷、高效地捕获目标分子以及实现磁性分离。本产品可配套自动化设备进行高通量操作。

■产品信息

尺寸: 200 nm

浓度: 10 mg/ml

配基: Streptavidin 链霉亲和素

磁性: 超顺磁

包覆材料： GMA

磁分离时间30S

储存：Store at 2-8°C（up to 6 months）in Storage Buffer (25 mM tris-HCl, 0.15 M NaCl, 0.05% tween20, 0.09% NaN3, pH 7.2). Do not freeze the reagent.

■结合能力

50 μg Streptavidin/mg beads;

4000 pmol free biotin/mg beads;

300 pmol biotin-oligonucleotide/mg beads;

20 pmol biotin-dsDNA fragments/mg beads;

本产品可完全替代市场主流链霉亲和素磁珠如GESera-Mag? Magnetic Beads Streptavidin、Pierce Streptavidin Magnetic Beads、Dynabeads? MyOne? 链酶亲和素、JSR的Streptavidin MS150等产品，表现为等同甚至更优的效果。

■质量控制

结合能力：每批链霉亲和素磁珠均用游离生物素测试保证的最小结合能力。

蛋白质的非特异性结合：将2 mg SA磁性颗粒与0.2 mg IgG在200 μl的50 mM磷酸盐缓冲液（0.15M NaCl，pH7.2）中温育1小时（15-25℃）。用2×200 ul温育缓冲液洗涤后，测上清液中的蛋白质浓度（A280）。未检测到非特定的结合。

无DNA酶活性：将1 μg的DNA底物于37℃下在含有1mg的 SA磁珠的100ml缓冲液M （10mM Tris-HCl，10mM MgCl2，50mM NaCl，1mM DTT，pH7.5）中温育4小时。未检测到DNase活性。

无RNA酶活性：将5ug MS2 RNA在37℃下在100ml 10mM Tris-HCl，pH7.5和1mg SA磁性颗粒中温育4小时。未检测到RNase活性。

无蛋白酶活性：将10mg的 SA磁珠与casein-resorufin在200μl的0.2M Tris-HCl（20mM CaCl2，pH 7.8）中于37℃温育30分钟。 TCA沉淀后，蛋白酶活性与上清液中574nm处的吸光度相关。没有检测到蛋白酶活性。

■如何使用链霉亲和素磁珠

链霉亲和素磁珠 G-streptavidin可以用于捕获生物素biotin标记的抗体、蛋白质、多肽、DNA等，可分为直接捕获与间接捕获两种方式。如下所示：

■链霉亲和素磁珠应用示例

例1. 生物素标记的核酸与SA磁珠的结合

例2. 链霉亲和素磁珠固定化生物素化抗体用于化学发光

例3.链霉亲和素磁珠研究DNA/RNA与蛋白互作

链霉亲和素磁珠详细实验方案见产品说明书，产品说明书请向客服索取。

■部分引用该磁珠文献

1. X. Li, C. Zhao, Y. Liu, Y. Li, F. Lian, D. Wang, Y. Zhang, J. Wang, X. Song, J. Li, Y. Yang, K. Xu, Fluorescence signal amplification assay for the detection of B. melitensis 16M, based on peptide-mediated magnetic separation technology and a AuNP-mediated bio-barcode assembled by quantum dot technology, Analyst (2019), 8 （链霉亲和素磁珠PuriMag G-Strep偶联生物素修饰多肽检测致病菌B. melitensis 16M）

2. Yuxi Wei, Yue Wang, Jiawei Wang, Xiaolin Yang, Honglan Qi, Qiang Gao, Chengxiao Zhang . Homogeneous electrogenerated chemiluminescence immunoassay for the detection of biomarkers by magnetic preconcentration on a magnetic electrode, Analytical and Bioanalytical Chemistry，July 2019, Volume 411, Issue 18, pp 4203–4211（链霉亲和素磁珠PuriMag G-Strep/ Homo-Strep偶联生物素修饰抗体，免疫电化学荧光检测生物标志物）

3. Chuchu Wang Xiaoyan Du Tiantian Xie Hao Li. Label‐ and modification‐free‐ased in situ selection of bovine serum albumin specific aptamer, journal of separation Science. First published: 24 September 2019 （链霉亲和素磁珠PuriMag G-Strep用于适配体筛选）

4. Hao Kong, Wei-wen Liu, Wei Zhang, Qiang Zhang, Cun-huai Wang, Muhammad Idrees Khan, Yu-xing Wang*, Liu-yin Fan*, and Cheng-xi Cao*. Facile, Rapid, and Low-Cost Electrophoresis Titration of Thrombin by Aptamer-Linked Magnetic Nanoparticles and a Redox Boundary Chip, ACS Appl. Mater. Interfaces, 2019, 11, 33, 29549–29556（链霉亲和素磁珠PuriMag G-Strep用于DNA适配体偶联）

5. Xiao-Li Chen, Hao Zheng, Wen-Ge Li, You-Hong Zhong, Xiao-Ping Chen, Jin-Xing Lu. Direct blood culturing of Candida spp. on solid medium was achieved by a rapid enrichment method with magnetic beads coated with rhMBL, Journal of Clinical Microbiology, 2020（链霉亲和素磁珠PuriMag Homo-Strep 纯化抗体）

6. Yang Feng , Neng-Bin Xie , Wan-Bing Tao , Jiang-Hui Ding , Xue-Jiao You , Cheng-Jie Ma , Xiaoxue Zhang , Chengqi Yi , Xiang Zhou , Bi-Feng Yuan , and Yu-Qi Feng. Transformation of 5-Carboxylcytosine to Cytosine Through C–C Bond Cleavage in Human Cells Constitutes a Novel Pathway for DNA Demethylation, CCS Chem. 2020, 2, 994–1008 (链霉亲和素磁珠PuriMag G-Strep用于biotin修饰DNA)

7. Haiping Wu, Min Zhao, Jia Li, Xiaoyan Zhou, Tiantian Yang, Dezhang Zhao, Ping Liu, Huangxian Ju, Wei Cheng*, and Shijia Ding*, Novel Protease-Free Long-Lasting Chemiluminescence System Based on the Dox-ABEI Chimeric Magnetic DNA Hydrogel for Ultrasensitive Immunoassay, ACS Applied Materials Interfaces 2020, 12, 42, 47270-47277 (链霉亲和素磁珠PuriMag G-Strep用于biotin修饰DNA)

8. Luyao Shen, Keke Jia, Tao Bing*, Zhibao Zhang, Xiaoxiao Zhen, Xiangjun Liu, Nan Zhang, and Dihua Shangguan*. Detection of Circulating Tumor-Related Materials by Aptamer Capturing and Endogenous Enzyme-Signal Amplification, Anal. Chem. 2020, 92, 7, 5370–5378 (链霉亲和素磁珠PuriMag G-Strep用于biotin修饰适配体)

9. Shanshan Yu, Limei Ai, Wei Wei, circRNA circ-MYBL2 is a novel tumor suppressor and potential biomarker in multiple myeloma, Human Cell, volume 34, pages219–228(2021) (链霉亲和素磁珠PuriMag G-Strep用于biotin修饰RNA pull down)

10. Xi Rao Lingling Lai Xiaopeng Li Liang Wang Ai Li Qian Yang. N6‐methyladenosine modification of circular RNA circ‐ARL3 facilitates Hepatitis B virus‐associated hepatocellular carcinoma via sponging miR‐1305, IUBMB LIFE, Volume73, Issue2, February 2021, Pages 408-417(链霉亲和素磁珠PuriMag G-Strep用于biotin修饰RNA pull down)

11. Hongwei Hu , Yujing Ding , Zihan Gao , Hao Li. S1 nuclease digestion-based rational truncation of PD-L1 aptamer and establishment of a signal dual amplification aptasensor, Sensors and Actuators B Chemical, 2021, 331:129442, DOI:10.1016/j.snb.2021.129442(链霉亲和素磁珠偶联抑制因子筛选适配体)

12. Qing An, Zipeng Zhou, Yi Xie, Yu Sun, Haixiang Zhang Yang Cao (2021)Knockdown of long non-coding RNA NEAT1 relieves the inflammatory response of spinal cord injury through targeting miR-211-5p/MAPK1 axis, Bioengineered, 12:1, 2702-2712, DOI:10.1080/21655979.2021.1930925 (链霉亲和素磁珠PuriMag G-Strep用于biotin修饰RNA pull down)

13. Dongyue Hu, Shusen Xiao, Qiaqia Guo, Rongrong Yue, Demin Geng and Debin Ji, Luminescence method for detection of aflatoxin B1 using ATP-releasing nucleotides, DOI: 10.1039/D1RA03870B (Paper) RSC Adv., 2021, 11, 24027-24031 (链霉亲和素磁珠PuriMag G-Strep用于biotin修饰适配体)

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